buffers
Resistentes a alterações de pH, os tampões protegem as amostras quando são adicionados ácidos ou bases. Disponíveis em pó e líquido, estes tampões rastreiam as proteínas de forma eficiente durante o isolamento e a separação por eletroforese. Também podem ser utilizados para calibrar e padronizar os medidores de pH, garantindo leituras precisas. As substâncias bloqueadoras impedem a ligação dos anticorpos aos locais livres na membrana, que produziriam um sinal de fundo interferente. Disponíveis em diversas concentrações para satisfazer qualquer necessidade, os tampões podem ser encontrados em formatos líquidos ou desidratados.
RoboSep™ Buffer 2
Supplier: Stemcell Technologies
RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.
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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Fixation Buffer
Supplier: Stemcell Technologies
Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.
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Intracellular Permeabilisation Buffer (10X)
Supplier: Stemcell Technologies
Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.
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RoboSep™-C PBS/EDTA Buffer
Supplier: Stemcell Technologies
PBS buffer containing EDTA.
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals
#: 20060.320
Supplier: VWR Chemicals