buffers

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: G-Biosciences

Solution developed to unmask antigens in paraffin embedded tissue sections. 

Supplier: Promega

Buffer used with T4 DNA Ligase to join cohesive or blunt ends.

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

Supplier: WTW

Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.

Supplier: Promega

The Reporter Lysis Buffer (RLB) allows β-gal and luciferase assays to be performed on cell lysates of cells co-transfected with vectors carrying these reporter genes.

Supplier: REAGECON

Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 25 °C, Plastic bottle

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).

Supplier: Promega

Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid lysis (within 5 minutes) of cultured mammalian cells without scraping or performing freeze-thaw cycles.

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Supplier: Promega

Lumit® dsRNA Assay Buffer (5X)

Supplier: REAGECON

Colour coded pH buffer solutions for the calibration of pH meters.

Supplier: Promega

Designed for use with T4 Polynucleotide Kinase to catalyze the transfer of the γ-phosphate from ATP to the 5´-terminus of poly- or mononucleotides bearing a 5´-hydroxyl group.

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

Supplier: WTW

Adapted to different electrode types for calibration and maintenance.