buffers

Supplier: WTW

Every bottle has its own lot reference with the expiration date.

Supplier: Thermo Fisher Scientific

1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

Supplier: Promega

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Promega

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: MP Biomedicals

A peroxidase protective buffer which stabilises HRP conjugates. It has 500 ml of PBS, pH 7,2, containing 0,5% BSA, antimicrobial preservatives and proprietary stabilising compounds.

Supplier: Promega

Running Buffer, TAE buffer solution 40X concentrate (TRIS-acetate-EDTA buffer)

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

Supplier: Promega

The 4-CORE® buffer pack contains convenient aliquots of restriction enzyme 10X Buffers A, B, C and D. The majority of restriction enzymes have optimal activity in one of these four 10X reaction buffers.

Supplier: Promega

Terminal transferase 5X buffer

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

Supplier: Mettler - Toledo

TISAB III solution for fluoride determinations

Supplier: Promega

RNA lysis buffer (RLA) is used with several RNA purification systems to lyse cells.

Supplier: Promega

The MULTI-CORE™ buffer pack contains convenient aliquots of the universal restriction enzyme 10X buffer. It is formulated to provide simple buffering conditions for performing multiple digestions.

Supplier: Promega

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.