buffers

Supplier: VWR Chemicals

A solução RapidBlock™ reduz o período de bloqueio para cinco minutos nos procedimentos de Western blotting e dot blotting, em membranas de PVDF e nitrocelulose. Esta formulação isenta de proteínas minimiza a reatividade cruzada e a ligação de anticorpos não-específicos, gerando blots com baixo ruído de fundo e uma melhor relação sindal/ruío.

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

Supplier: Promega

Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed to promote rapid lysis (within 5 minutes) of cultured mammalian cells without scraping or performing freeze-thaw cycles.

Supplier: Promega

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

Supplier: G-Biosciences

NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.

Supplier: Honeywell Chemicals

TraceSELECT™, for flame atomic absorption spectrometry.

Supplier: Promega

Lumit® dsRNA Assay Buffer (5X)

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

Supplier: REAGECON

Colour coded pH buffer solutions for the calibration of pH meters.

Supplier: Thermo Fisher Scientific

Used in Western blotting protocols.

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)

Supplier: Merck

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: Merck

Washing buffer, PBS buffer solution (phosphate buffered saline) pH 7,4