buffers

Supplier: Thermo Fisher Scientific

Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

Supplier: NOVILYTIC

5 sealed salt packets specifically formulated for use with Proteometer-CV kit.

Supplier: HyClone products (Cytiva)

A balanced salt solution tested for use with embryonic stem cells.

Supplier: NOVILYTIC

7 vials of the Proteometer-L fluorescent reagent specifically formulated for use with Proteometer-L kit.

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-L Starter Kit.

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

Supplier: Thermo Fisher Scientific

Liquid.

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: NOVILYTIC

1 sealed salt packet specifically formulated for use with Proteometer-CV kit.

Supplier: NOVILYTIC

3 vials of Proteometer-UFT Fluorescent reagent specifically formulated for use with Proteometer-UFT kit.

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

Supplier: NOVILYTIC

Fluorescent reagent for the Proteometer-CV kit.

Supplier: Thermo Fisher Scientific

Use directly to re-suspend and/or dilute purified DNA or RNA.

Supplier: Thermo Fisher Scientific

HEPES buffer solution pH 7.3 (1 mol/l)

Supplier: Thermo Fisher Scientific

10X TAE solution consisting of 400 mM Tris and 0,01 M EDTA in high purity dH₂O adjusted to pH 8,3. Solution is 0,2 μm filtered and dispensed into durable, square Nalgene® bottles.