"Stemcell Technologies"

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD41, APC-conjugated.

Supplier: Stemcell Technologies

Fibroblast growth factor 8B (FGF-8B) is a member of the fibroblast growth factor (FGF) family and is an isoform of FGF-8. Cytokines in the FGF family possess broad mitogenic and cell survival activities (Folkman and Klagsbrun; Kimelman and Kirschner) and are involved in a variety of biological processes, including cell proliferation, differentiation, survival, and apoptosis (Folkman and Klagsbrun; Klagsbrun; Rifkin and Moscatelli). FGF-8B signals through FGF receptors (FGFRs) to activate PI3K and MAPK pathways. FGF-8B regulates gastrulation, epithelial-mesenchymal transition, and mesenchymal to epithelial differentiation during embryonic development. FGF-8B has also been found in peripheral blood leukocytes and healthy bone marrow samples (Mattila and Härkönen). FGF-8B has mitogenic effects on somatic cells in the germinal epithelium and is expressed in adult mouse ovarian cells and tissues, which suggests that it regulates maturation of oocytes and seminiferous epithelium in testes (Valve et al.). This product is animal component-free.

Supplier: Stemcell Technologies

Mouse monoclonal IgM antibody against human, chimpanzee CD66b, Pacific Blue™-conjugated.

Supplier: Stemcell Technologies

Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that regulates a broad variety of developmental functions. After LIF binds to LIF receptor (LIFR), LIFR associates with gp130 and activates JAK/STAT and MAPK signaling (Auernhammer and Melmed; Suman et al.). LIFR activation of STAT3 is essential for maintaining the mouse embryonic stem cell phenotype (Niwa et al.). Produced by the endometrium, LIF plays an important autocrine and paracrine role in implantation by regulating proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment (Auernhammer and Melmed; Suman et al.). Human LIF can be used for the maintenance of mouse embryonic stem cells, however mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive (Dahéron et al.). LIF is produced by CD4+ and activated regulatory T cells, and promotes Foxp3 expression, while repressing Th17 lineage-specific genes (Metcalfe). LIF is also secreted by mesenchymal stromal cells, where it supports hematopoiesis and immune modulation (Nasef et al.). This product is animal component-free.

Supplier: Stemcell Technologies

Immunomagnetic negative selection of human pan-B cells

Supplier: Stemcell Technologies

The SARS-CoV-2 (Nucleocapsid protein) Peptide pool is a lyophilised mixture of 102 peptides from the nucleocapsid protein of SARS-CoV-2. Nucleocapsid protein is one of four structural proteins that interacts with RNA to form the nucleocapsid. It also interacts with membrane protein (protein M) in the packaging of positive-strand viral genome RNA during virion assembly. The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 419 on SARS-CoV-2 nucleocapsid protein.

Supplier: Stemcell Technologies

Ready-to-use marker dye for loading DNA samples into agarose gel wells and tracking migration during electrophoresis.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee CD45, unconjugated.

Supplier: Stemcell Technologies

Rat monoclonal IgG2b antibody against mouse CD117 (c-Kit), unconjugated.

Supplier: Stemcell Technologies

For guide RNA generation in CRISPR-Cas9 genome editing.

Supplier: Stemcell Technologies

Interleukin 15 (IL-15) is a four-alpha helix bundle cytokine with many similar properties to IL-2. The IL-15 receptor is a heterotrimeric receptor composed of IL-15Ra (the high-affinity receptor for IL-15), as well as IL-2/15Rb (CD122) and common gamma chain (CD132). IL-15 binds to IL-15Rα receptor and can then be presented in trans to IL-2/15Rb and common gamma chain on other cells. Trans-presentation is thought to be the major mechanism by which IL-15-mediated responses occur in mice, although may not be necessary in humans (Castillo et al.). The cytoplasmic domains of IL-2/15Rb and common gamma chain mediate signaling to activate JAK/STAT and PI3K pathways. IL-15 supports the survival and proliferation of naïve CD4+ and CD8+ T cells, and promotes homeostasis of memory T cells. IL-15 also promotes the survival and differentiation of NK cells and regulates their cytolytic activity (Ma et al.). This product is animal component-free.

Supplier: Stemcell Technologies

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including mast cells, basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Fung et al.; Metcalf et al.; Broughton et al.). IL-3 is produced by activated T cells, and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by mast cells, basophils and eosinophils. The mouse IL-3 receptor consists of a unique alpha-subunit (CD123) and two beta subunits, one specific for IL-3 (βIL-3), the other shared with the receptors for IL-5 and GM-CSF (beta common chain, βc or CD131). IL-3 binding to heterodimeric receptors containing the alpha subunit and one of either beta subunits activates JAK/STAT, MAPK, and PI3K signaling pathways (Scott and Begley).

Supplier: Stemcell Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD41, FITC-conjugated.

Supplier: Stemcell Technologies

Hamster (Syrian) monoclonal antibody against mouse KLRG1, PE-conjugated.

Supplier: Stemcell Technologies

Rat monoclonal IgG2b antibody against mouse CD4, FITC-conjugated.

Supplier: Stemcell Technologies

Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD14, FITC-conjugated.