2418 Results for: "STEMCELL TECHNOLOGIES"

Supplier: STEMCELL TECHNOLOGIES

Hamster (Syrian) monoclonal antibody against mouse KLRG1, APC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Supplement (50X) without vitamin A for the culture of neural progenitor cells.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal antibody against human IFN-gamma, FITC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal antibody against mouse CD45.2, APC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Rat monoclonal antibody against human IL-4, unconjugated.

Supplier: STEMCELL TECHNOLOGIES

Rabbit polyclonal antibody against human, mouse PAX6 (AN).

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG2b, antibody against human, chimpanzee, CD45RA, APC-Cyanine7-conjugated.

Supplier: STEMCELL TECHNOLOGIES

A solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10 mM.

Supplier: STEMCELL TECHNOLOGIES

Serum-free differentiation kit for the generation of hepatocyte-like cells from human PSCs.

Supplier: STEMCELL TECHNOLOGIES

cGMP, stabilised feeder-free maintenance medium for human ES and iPS cells.

Supplier: STEMCELL TECHNOLOGIES

For automated cell isolation with RoboSep™-C

Supplier: STEMCELL TECHNOLOGIES

Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells

Supplier: STEMCELL TECHNOLOGIES

Immunodensity isolation of untouched T cells.

Supplier: STEMCELL TECHNOLOGIES

Serum-free medium for culture and expansion of hematopoietic cells.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD138 (syndecan-1), APC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Immunomagnetic isolation of untouched mouse pan-naïve T cells

Supplier: STEMCELL TECHNOLOGIES

Immunomagnetic isolation of untouched human CD4+ T cells.

Supplier: STEMCELL TECHNOLOGIES

Immunomagnetic positive selection of CD90.2+ cells from mouse splenocytes or other tissues.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO, PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1 antibody against human CD56 (NCAM), PE­conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1 antibody against human, chimpanzee, horse CD38, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4), PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD90 (Thy-1), PE-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2, APC-conjugated.

Supplier: STEMCELL TECHNOLOGIES

Epidermal growth factor (EGF) is characterized by high affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter and Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright et al.). Central nervous system stem cells also proliferate in response to the EGF stimulus (Reynolds and Weiss).

Supplier: STEMCELL TECHNOLOGIES

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the tumor growth factor (TGF)-beta superfamily. The GDNF family of growth factors also includes neurturin, persephin, and artemin, which have seven conserved cysteine residues called cysteine-knots (Treanor et al.). GDNF family ligands signal through binding to specific GDNF-family receptor-α (GFRα) co-receptors and activate the RET receptor tyrosine kinase (Durbec et al.). Four different forms of GFRα co-receptors have been characterized (GFRα 1-4); GDNF binds specifically to GFRα1 prior to forming a complex with RET (Airaksinen and Saarma). GDNF is known to promote survival and morphological differentiation of midbrain dopaminergic neurons in both in vivo and in vitro studies and increases their high-affinity dopamine uptake (Granholm et al.; Lin et al.). GDNF has also been shown to have restorative effects on dying dopaminergic neurons in response to degenerative toxins (Aoi et al.). GDNF, together with Human Recombinant BDNF (brain-derived neurotrophic factor), BrainPhys™ Neuronal Medium, and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.).

Supplier: STEMCELL TECHNOLOGIES

Platelet-derived growth factor (PDGF) is a dimeric glycoprotein consisting of two disulfide bridge stabilized polypeptide chains, A and B, which are assembled as heterodimers (PDGF-AB) or homodimers (PDGF-AA and PDGF-BB) (Fretto et al.; Westermark and Heldin). PDGF signals through the receptor tyrosine kinases PDGFRalpha and PDGFRbeta. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin- like fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). PDGF-BB is secreted by osteoblasts to induce mesenchymal stem cell migration and angiogenesis. It has also been shown that PDGF-BB is secreted by preosteoclasts during bone modeling and remodeling to induce angiogenesis and thus proper osteogenesis (Xie et al.).

Supplier: STEMCELL TECHNOLOGIES

Macrophage colony-stimulating factor (M-CSF) is a homodimeric glycoprotein growth factor that regulates proliferation and differentiation of myeloid hematopoietic progenitor cells to mononuclear phagocytic cell lineages, including monocytes, macrophages, and osteoclasts. M-CSF is a crucial factor for the development of tissue-resident macrophages in most tissues (Ginhoux and Jung). It is required for the maturation and activation of monocytes and macrophages, and regulates inflammatory responses in conjunction with other stimuli such as IFN-γ, LPS, and IL-4 (Murray et al.). M-CSF is also required for bone resorption by osteoclasts, and is involved in the development and regulation of the placenta, mammary gland, and brain. M-CSF is produced by monocytes, fibroblasts, osteoclasts, stromal cells, endothelial cells, and tumor cells (Chockalingam and Ghosh). M-CSF exerts its biological effects by signaling through a receptor tyrosine kinase (CSF-1R or M-CSF-R) encoded by the c-fms proto-oncogene (Hamilton). CSF-1R shares similar structural features with other growth factor receptors, including the stem cell factor (SCF) receptor, platelet-derived growth factor receptor (PDGF-R), and Flt3/Flk-2 receptor tyrosine kinase. Stimulation of the CSF-1R upon binding to M-CSF activates MAPK, PI3K, and PLCγ signaling pathways (Chockalingam and Ghosh). Human and mouse M-CSF sequences are highly conserved both at nucleotide and amino acid levels (80% homology; DeLamarter et al.).

Supplier: STEMCELL TECHNOLOGIES

Brain-derived neurotrophic factor (BDNF), like nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4), is a member of the NGF family of neurotrophins, which are required for the differentiation and survival of specific neuronal subpopulations in both the central and the peripheral nervous systems (Minichiello and Klein; Minichiello et al.). BDNF binds with high affinity to the tropomyosin receptor kinase B (TrkB), and activates AKT and ERK pathways (Mattson et al.). It is expressed in the hippocampus, cortex, and synapses of the basal forebrain. BDNF acts as a survival factor for human embryonic stem cells when plated on either feeder cells or Corning® Matrigel® (Pyle et al.). BDNF regulates synaptic transmission and plasticity at adult synapses in the central nervous system, and contributes to adaptive neuronal responses including long-term potentiation, long-term depression, certain forms of short-term synaptic plasticity, and homeostatic regulation of neuronal excitability (Reichardt). It also has a role in neurogenesis by promoting survival and growth of dorsal root ganglion cells, and hippocampal and cortical neurons (Binder and Scharfman). BDNF, together with glial cell line-derived neurotrophic factor (GDNF) and other supplements, is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Brafman).

Supplier: STEMCELL TECHNOLOGIES

RANTES (regulated upon activation, normal T cell expressed and secreted), also known as CCL5, is a member of the CC family of chemokines and is able to recruit leukocytes to sites of inflammation (Schall et al.). RANTES is secreted by T lymphocytes, macrophages, platelets, synovial fibroblasts, tubular epithelium, and certain types of tumor cells (Aldinucci and Colombatti; Soria and Ben-Baruch). This chemokine exerts its effect by interacting with the chemokine receptors CCR1, CCR3, CCR4, and CCR5. RANTES plays an active role in recruiting a variety of leukocytes into inflammatory sites, including T cells, macrophages, eosinophils, and basophils. In collaboration with certain cytokines that are released by T cells such as IL-2 and IFN-γ, RANTES also induces the activation and proliferation of NK cells to generate CC chemokine-activated killer cells, which are highly cytolytic (Lv et al.; Maghazachi et al.). It has been shown that RANTES produced by CD8+ T cells inhibits HIV infection of primary human peripheral blood mononuclear cells (Appay and Rowland-Jones; Cocchi et al.).