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Alcian blue 8GX, certified, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian blue 8GX, certified, Sigma-Aldrich®

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Fuchsin (acid) for tissue, stain, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Fuchsin is mainly used as a diachrome and is part of the connective tissue stains. It also works as a pH indicator.

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Safranine O ≥85% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in gram staining.

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Light green SF yellowish, powder or crystals, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Light green SF yellowish (LGSFY) is a disodium salt of an acid triphenylmethane dye. It is also known as Light Green, Lissamine Green SF, Food Green 2, Green No. 205, Acid Green, Acid Brilliant Green 5, and Acid Brilliant Green SF. LGSFY is an important component of Papanicolaou stains in addition to eosin Y and bismark brown Y. Light green SF yellowish is a histological stain for collagen and a permitted colorant for foodstuffs and beverages.

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Chlorophyllin, coppered trisodium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Chlorophyllin sodium copper salt has been used to study the effect of chlorophyllin against radiation-induced hematopoietic syndrome. It has been used to study the effect of chlorophyllin on the excretion of PhIP

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Estriol 3-sulphate sodium salt ≥98% (by TLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Estriol 3-sulfate sodium salt, (1,3,5(10)-Estratriene-3,16alpha,17beta-triol 3-sulfate; 3,16alpha,17beta-Trihydroxy-1,3,5(10)-estratriene 3-sulfate)

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Peroxidase (Myeloperoxidase) Leukocyte Kit Hanker-Yates substrate kit, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Staining system for polymorphonucleocytes in blood or bone marrow films.

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Tissue freezing medium, PolyFreeze, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tissue samples may be snap frozen using PolyFreeze with isopentane and liquid nitrogen, dry ice (slush/slurry or bunker), or in a cryostat. PolyFreeze media do not cause autofluorescence and can easily be washed away during tissue fixation when rinsed prior to staining. Once rinsed off the slide, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.

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Orange G, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Orange G, Sigma-Aldrich®

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Carbol fuchsin solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in the Acid Fast Stain, Procedure No. HT80 with Malachite Green counterstain Catalog No. HT80-2

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Eosin Y ∼99% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

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Haematoxylin (Harris), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.

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Chromazurol B

Supplier: SIGMA ALDRICH MICROSCOPY

Chromeazurol B is a dye, belonging to the class of triphenylmethanes.

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Ponceau Xylidine ≥60 % (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau Xylidine has been used as part of the Goldner’s trichome stain to stain thin sections. It has also been used to prepare Fuchsin-Ponceau solution to stain an amyotrophic lateral sclerosis tissue-engineered

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N,N-Dimethyl-1,4-phenylenediammonium dichloride ≥99.00% (by titrimetric analysis) suitable for peroxidase analysis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) has been used in the HID-AB (high iron diamine-alcian blue) staining of intestinal tract samples. N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) is used for the determination of peroxidase. DMPD is converted into a red pigment of semiquinone nature. It is also used in the HID-AB (high iron diamine-alcian blue) staining procedures, which are used to differentiate between sialomucin and sulfomucin in the gastrointestinal tract.

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Calcein AM 4 mM in DMSO ≥90% (by HPCE), clear solution

Supplier: SIGMA ALDRICH MICROSCOPY

Calcein AM 4 mM in DMSO ≥90% (by HPCE), clear solution

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Melanin 99% (by TLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Melanin is a ubiquitous and prominent pigment responsible for color of skin, hair and eyes. Melanin is classified into eumelanin and pheomelanin. Eumelanin is black to dark brown pigment and is present in black hair and retina of the eye. Pheomelanin is yellow to reddish brown pigment present is red hair and red feathers. Melanins are high molecular weight pigments synthesised from phenolic or indolic precursors and is widely present in animals, plants and microorganisms.

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Dextran solution 20% (from Leuconostoc mesenteroides), autoclaved, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Use of dextrans as long and hydrophilic spacer arms to improve the performance of immobilised proteins acting on macromolecules.

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Acriflavine fluorescent labeling reagent, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.

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Lucifer yellow CH dilithium salt for fluorescence Stain, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

A highly fluorescent dye, useful in marking nerve cells.

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Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt,powder (Sulfo-SMCC)

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Fluorinert™ FC-770, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorinert™ belongs to the group of aliphatic compounds. It is considered as a pressure-transmitting medium because it is highly inert. Fluorinert™ Electronic Liquid FC-770 is a thermally stable, fully-fluorinated liquid that has ideal properties for use as a heat transfer fluid in a variety of industries. The inertness of Fluorinert™ FC-770 permits its use as a direct contact single and two-phase coolant in certain supercomputers and sensitive military electronics. Its high dielectric strength and electrical resistivity are ideal for applications in high voltage transformers and power electronics. In the semiconductor manufacturing industry, its wide liquid range makes Fluorinert™ liquid FC-770 suited for cooling ion implanters, dry etchers and CVD machines. Its low pour point also permits its use in thermal shock and test equipment.

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Tuerk solution for microscopy, for leukocyte count, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Counting leukocytes in the counting chamber.

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Acid Yellow 25 40% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Yellow 25, Dye content 40 %

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meso-Tetraphenylporphine 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

5,10,15,20-Tetraphenyl-21H, 23H-porphine is a porphyrin compound.

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Nuclear Fast Red, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Nuclear fast red dye has anthraquinonic nature and is used either alone or in combination with other dyes. It is mainly used for staining of nuclei.

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2',4'-Dihydroxy-3'-methylacetophenone 90%, Technical Grade, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2',4'-Dihydroxy-3'-methylacetophenone 90%, Technical Grade, Sigma-Aldrich®

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Tetrabromophenol Blue sodium salt 85% (dye content) ≥84.5% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tetrabromophenol Blue sodium salt, Dye content 85% (3,3',3'',4,5,5',5'',6-Octabromophenolsulfonphthalein sodium salt).

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3,6-Dibutoxy-1,2-benzenedicarbonitrile ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3,6-Dibutoxy-1,2-benzenedicarbonitrile,97% (3,6-Dibutoxyphthalonitrile)

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Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

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