2677 Results for: "Promega"

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

(T/C)TG at 37 °C in its own unique buffer.

Supplier: Promega

CCGG G at 37 °C in its own unique buffer.

Supplier: Promega

The Maxwell® 16 Blood DNA Purification Kit is designed for automated extraction of DNA from whole blood or buffy coat samples on the Maxwell® 16 instrument. The kit is optimised to process a wide range of volumes of fresh or stored human blood as well as animal blood samples. Up to 400 µl of whole blood can be processed to yield up to 15 µg of gDNA from human blood, depending on white blood cell (WBC) count.

Supplier: Promega

The P450-Glo CYP2D6 assays provide a homogeneous, luminescent method for measuring cytochrome CYP2D6 activity.

Supplier: Promega

The 10 bp DNA Step Ladder has ten blunt-ended DNA fragments ranging from 10 bp to 100 bp in exactly 10 bp increments. These ladders have defined sizes with exact incremental steps between bands.

Supplier: Promega

PCR markers have six bands of equal intensity of 50, 150, 300, 500, 750 and 1000 bp.

Supplier: Promega

CellTiter 96® Non-radioactive Cell Proliferation Assay is a collection of qualified reagents that provide a convenient method of determining viable cell number. The CellTiter 96® Assay is a modification of the MTT assay method described by Mosmann and incorporates several improvements to the method that address previous technical problems, including serum protein precipitation caused by adding organic solvent, interference by phenol red, incomplete solubilisation of the formazan crystals resulting in lower sensitivity, and stability of the coloured product.

Supplier: Promega

Murine 2,5S nerve growth factor (2,5S mNGF) is a 26 kDa protein composed of two identical 118 amino acid chains.

Supplier: Promega

The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: Promega

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minutes signal half-life without prior sample processing.

Supplier: Promega

Formamide is often used for the denaturation of nucleic acids in applications such as hybridization, sequencing gel electrophoresis and electron microscopy.

Supplier: Promega

The 200 bp DNA Step Ladder has 33 blunt-ended DNA fragments ranging from 200 to 6600 bp in 200 bp increments. These ladders have defined sizes with exact incremental steps between bands.

Supplier: Promega

Anti-TUBB3 Mouse Monoclonal Antibody [clone: 5G8]

Supplier: Promega

The Wizard® MagneSil® plasmid DNA purification system provides a simple and reliable method for the rapid isolation of plasmid DNA in a 96-well, high throughput format. The purified plasmid can be used directly for automated fluorescent sequencing, such as with BigDye® terminator sequencing chemistry, as well as for other standard molecular biology techniques including restriction enzyme digestion. The use of the MagneSil® paramagnetic particles for lysate clearing (BLUE) as well as DNA capture (RED) circumvents the need for centrifugation or vacuum manifolds, making the system ideal for full automation on a Beckman Coulter or Tecan instrument.

Supplier: Promega

BenchTop 100 bp DNA Ladder with 11 fragments that range in size from 100 to 1000 bp in 100 bp increments with an additional band at 1500 bp. The 500 bp fragment is present at increased intensity for easy identification. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide kinase, allowing visualisation by autoradiography.

Supplier: Promega

The pro-luciferin substrates can be used to monitor the activity of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of the substrate.
The luciferin derivative, 2-hydroxyethyl 2-(6-(3,3-dimethoxypropoxy)benzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylate, referred to as Luciferin-2J2/4F12, is converted to a luciferin ester, most prominently by CYP2J2 and to a lesser extent by CYP4F12, CYP1A1, CYP1B1 and CYP3A4. The Luciferin-2J2/4F12 substrate is selective for human CYP2J2, CYP4F12, CYP3A4, CYP1A1 and CYP1B1, and has a strong preference for CYP2J2 under conditions that favor activity of this enzyme.

Supplier: Promega

The QuantiFluor® RNA system contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution.

Supplier: Promega

ProTEV Plus is an improved 48 kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity.

Supplier: Promega

Quantus is a compact, dual-channel fluorometer designed to provide highly sensitive fluorescent detection, when quantifying nucleic acids.

Supplier: Promega

The CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity allowing more accurate determination of viability compared to other assay methods.

Supplier: Promega

GoTaq® Long PCR Master Mix contains the high performance GoTaq® Hot Start Polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase. This optimised enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA.

Supplier: Promega

High performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94 to 95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 °C for convenient, room-temperature reaction set-up.

Supplier: Promega

These whole-cell protein extract prepared from human K562 cells are designed for monitoring mass spec instrument performance.

Supplier: Promega

Diamond™ nucleic acid dye is a sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualise nucleic acids in gels. It is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system.

Supplier: Promega

ProteaseMAX™ Surfactant ensures fast and efficient protein digestion with proteases such as trypsin, chymotrypsin and Lys-C.

Supplier: Promega

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. It is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays.

Supplier: Promega

Herring sperm DNA is useful as a blocking agent in nucleic acid hybridisation experiments.

Supplier: Promega

The Wizard® SV 96 plasmid DNA purification systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica membrane, 96-well, high throughput format. A single plate can be processed in 60 minutes or less. The purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques, including restriction enzyme digestion.

Supplier: Promega

Nucleic Acid Purification Kits and Reagents, Neutralisation solution (NSA)