"Promega"

Supplier: Promega

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important.

Supplier: Promega

The Wizard® PCR preps DNA purification system provides a simple, reliable way to purify double-stranded PCR amplified DNA. This system also can be used to purify DNA fragments from agarose gels. The DNA can be eluted in water or TE buffer, free of salts or macromolecular contaminants. Multiple PCR preps may be processed easily at one time with the Vac-Man® naboratory vacuum manifold.

Supplier: Promega

ReliaPrep™ RNA Miniprep systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15 µl).

Supplier: Promega

The GoTaq® 2-Step RT-qPCR system facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components such as GoScript™ reverse transcription system and GoTaq® qPCR master mix. It includes an optimised reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Supplier: Promega

Agarose for applications that require recovery of intact DNA fragments after gel electrophoresis.

Supplier: Promega

The RealTime-Glo™ MT Cell Viability Assay is a non lytic, homogeneous, bioluminescent method to measure cell viability in real time using a simple, plate-based method. RealTime-Glo™ MT Cell Viability Assay determines the number of viable cells in culture by measuring the reducing potential of cells and thus metabolism (MT).

Supplier: Promega

High-quality deoxynucleotide triphosphates (dNTPs) are important for key procedures including cDNA synthesis, sequencing, cloning, and preparation of libraries for next generation sequencing.

Supplier: Promega

Agarose

Supplier: Promega

The PureYield™ plasmid Maxiprep system is designed to isolate transfection quality plasmid DNA. The system provides a rapid method for purification up to 1 mg of plasmid DNA from a 250 ml bacterial culture.

Supplier: Promega

The pGL3 luciferase reporter vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene
expression. These may be cis- or trans-acting factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, with a modified coding region for firefly (Photinus pyralis) luciferase that has been optimised for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterisation of the putative regulatory sequences under investigation.

Supplier: Promega

The Mitochondrial ToxGlo™ Assay is a cell-based assay method that employs a sequential addition, multiplexed assay chemistry for predicting potential mitochondrial dysfunction as a result of xenobiotic exposure.

Supplier: Promega

The DeadEnd™ Colorimetric TUNEL system is a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay used to detect the apoptotic cells in situ at the single-cell level.

Supplier: Promega

Bisacrylamide, Molecular Grade, is a cross-linking agent used in the preparation of polyacrylamide gels. This product is tested for its efficiency in gel polymerization.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

Glu-C sequencing grade serine protease (S. aureus V8) specifically cleaves at the C-terminus of either aspartic or glutamic acid residues.

Supplier: Promega

The Wizard® Magnetic DNA Purification Kits for Food are used to isolate DNA from a variety of food samples like corn seeds, cornmeal, soybeans, soy flour and soy milk. Processed food, such as corn chips, chocolate and chocolate-containing foods, lecithin and vegetable oils can also be used with protocol variations.