"Promega"

Supplier: Promega

Used in many molecular biology and forensic applications.

Supplier: Promega

The Renilla-Glo® Luciferase Assay System is a single-addition reagent that generates a glow-type signal with Renilla luciferase. It has the capacity to lyse cells, reduce the auto luminescence of the coelenterazine substrate and produce a stable signal (half-life greater than 60 minutes at 22 °C).

Supplier: Promega

The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).

Supplier: Promega

Firefly Luciferase Substrate for Use in Animal Imaging Studies.

Supplier: Promega

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: Promega

The NanoBRET™ Nano-Glo® protein detection system is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc® luciferase as the BRET energy donor and HaloTag® protein labeled with the HaloTag® NanoBRET™ 618 fluorescent ligand as the energy acceptor to measure the interaction of two binding partners in live cells. The HaloTag® NanoBRET™ 618 ligand is added directly to the cells during plating, and the NanoBRET™ Nano-Glo® substrate is added to the sample just prior to measuring donor and acceptor emission.

Supplier: Promega

TA sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

Ribonucleotide triphosphates (rNTPs) compatible with the Riboprobe® and HeLaScribe® systems.

Supplier: Promega

The Wizard® SV genomic DNA purification system provides a fast, simple, membrane based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K. Amplifiable genomic DNA can be isolated from up to 5×10⁶ cells, 20 mg of tissue or up to 1,2 cm of a mouse tail tip without a centrifugation clearing step.

Supplier: Promega

These PvuI restriction enzyme recognises the cut site of CG AT^˅CG and GC_˄TA GC. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

Supplier: Promega

sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

These PvuII restriction enzyme recognises the cut site CAG^˅CTG and GTC_˄GAC. Star activity may be observed with glycerol concentrations >12% or with enzyme: DNA ratios >25 U/μg.

Supplier: Promega

T3 RNA Polymerase is a DNA-dependent RNA polymerase that synthesises RNA using only T3 DNA or DNA templates cloned downstream from a T3 promoter. The polymerase can incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates during transcription.

Supplier: Promega

The Reverse Transcription system provides reagents to efficiently reverse transcribe RNA into cDNA. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesises single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and random primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1 μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications.

Supplier: Promega

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

Supplier: Promega

Mouse genomic DNA is isolated from whole blood from disease-free mice.