"Promega"

Supplier: Promega

High performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94 to 95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 °C for convenient, room-temperature reaction set-up.

Supplier: Promega

PCR markers have six bands of equal intensity of 50, 150, 300, 500, 750 and 1000 bp.

Supplier: Promega

The CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity allowing more accurate determination of viability compared to other assay methods.

Supplier: Promega

Urea

Supplier: Promega

IPTG, Dioxane-Free (isopropyl-β-d-thiogalactopyranoside), is an inducer of β-galactosidase activity in many bacteria. Functioning as a lac analog, IPTG induces β-galactosidase activity by binding to and inhibiting the lac repressor. This product is used to differentiate recombinants from nonrecombinants in cloning strategies using vectors containing the lacZ or lacZ α-peptide gene..

Supplier: Promega

G418 disulphate

Supplier: Promega

The SP6 and T7 promoter primers are designed for sequencing inserts cloned into the pGEM® Vectors.

Supplier: Promega

CellTiter 96® Non-radioactive Cell Proliferation Assay is a collection of qualified reagents that provide a convenient method of determining viable cell number. The CellTiter 96® Assay is a modification of the MTT assay method described by Mosmann and incorporates several improvements to the method that address previous technical problems, including serum protein precipitation caused by adding organic solvent, interference by phenol red, incomplete solubilisation of the formazan crystals resulting in lower sensitivity, and stability of the coloured product.

Supplier: Promega

The Wizard® MagneSil® plasmid DNA purification system provides a simple and reliable method for the rapid isolation of plasmid DNA in a 96-well, high throughput format. The purified plasmid can be used directly for automated fluorescent sequencing, such as with BigDye® terminator sequencing chemistry, as well as for other standard molecular biology techniques including restriction enzyme digestion. The use of the MagneSil® paramagnetic particles for lysate clearing (BLUE) as well as DNA capture (RED) circumvents the need for centrifugation or vacuum manifolds, making the system ideal for full automation on a Beckman Coulter or Tecan instrument.

Supplier: Promega

CATG G sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and the 3´→5´ exonuclease activities present in intact E. coli DNA polymerase I.

Supplier: Promega

The pro-luciferin substrates can be used to monitor the activity of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of the substrate.
The luciferin derivative, 2-hydroxyethyl 2-(6-(3,3-dimethoxypropoxy)benzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylate, referred to as Luciferin-2J2/4F12, is converted to a luciferin ester, most prominently by CYP2J2 and to a lesser extent by CYP4F12, CYP1A1, CYP1B1 and CYP3A4. The Luciferin-2J2/4F12 substrate is selective for human CYP2J2, CYP4F12, CYP3A4, CYP1A1 and CYP1B1, and has a strong preference for CYP2J2 under conditions that favor activity of this enzyme.

Supplier: Promega

(T/C)TG at 37 °C in its own unique buffer.

Supplier: Promega

CCGG G at 37 °C in its own unique buffer.

Supplier: Promega

Formamide is often used for the denaturation of nucleic acids in applications such as hybridization, sequencing gel electrophoresis and electron microscopy.

Supplier: Promega

Pgp-Glo™ Assay Systems provide the necessary reagents to perform luminescent P-glycoprotein (Pgp) ATPase assays. Pgp, also known as MDR1 and ABCB1, is a 170 kDa integral plasma membrane protein that functions as an ATP-dependent drug efflux pump and plays an important role in multidrug resistance and certain adverse drug-drug interactions. Compounds that interact with Pgp can be identified as stimulators or inhibitors of its ATPase activity. Compounds that are substrates for transport by Pgp typically stimulate its ATPase activity.