2677 Results for: "Promega"

Supplier: Promega

5X Green GoTaq® buffer

Supplier: Promega

GoTaq® Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl₂ and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR.

Supplier: Promega

Pgp-Glo™ Assay Systems provide the necessary reagents to perform luminescent P-glycoprotein (Pgp) ATPase assays. Pgp, also known as MDR1 and ABCB1, is a 170 kDa integral plasma membrane protein that functions as an ATP-dependent drug efflux pump and plays an important role in multidrug resistance and certain adverse drug-drug interactions. Compounds that interact with Pgp can be identified as stimulators or inhibitors of its ATPase activity. Compounds that are substrates for transport by Pgp typically stimulate its ATPase activity.

Supplier: Promega

Cellular assay, Apo-ONE® Homogeneous Caspase-3/7 Buffer

Supplier: Promega

The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour.

Supplier: Promega

FuGENE® HD transfection reagent has a non-liposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in chemically defined media and does not contain any animal-derived components.

Supplier: Promega

The CytoTox 96® Non-Radioactive Cytotoxicity Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [⁵¹Cr] is released in radioactive assays.

Supplier: Promega

The λ DNA/EcoRI + HindIII Markers have 13 ethanol-precipitated DNA fragments ranging in size from 125 to 21226 bp.

Supplier: Promega

DNA Polymerase I Large (Klenow) Fragment is a 68kDa C-terminal fragment of E. coli DNA Polymerase I that lacks the 5´→3´ exonuclease activity of intact DNA polymerase I but retains its 5´→3´ polymerase, 3´→5´ exonuclease and strand displacement activities.

Supplier: Promega

DNA Polymerase I catalyses the template-directed polymerisation of nucleotides into duplex DNA in a 5´→3´ direction. DNA Polymerase I possesses a 3´→5´ exonuclease activity or 'proofreading' function, which lowers the error rate during DNA replication, and also contains a 5´→3´ exonuclease activity, which enables the enzyme to replace nucleotides in the growing strand of DNA by nick translation.

Supplier: Promega

The SP6 and T7 promoter primers are designed for sequencing inserts cloned into the pGEM® Vectors.

Supplier: Promega

DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus, is a DNA-dependent DNA polymerase that lacks both the 5´→3´ and the 3´→5´ exonuclease activities present in intact E. coli DNA polymerase I.

Supplier: Promega

G418 disulphate

Supplier: Promega

Wizard® Plus Megapreps DNA purification system provides a simple and rapid method for large scale purifications of plasmid DNA. This system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high copy number plasmid DNA (200 to 20000 bp) from a 1000 ml culture in less than 3 hours. The purified DNA can be used directly for DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions.

Supplier: Promega

PCR Nucleotide Mix is a premixed solution containing the sodium salts of dATP, dCTP, dGTP and dTTP, each at a concentration of 10mM or 25mM in water.

Supplier: Promega

SP6 RNA Polymerase is a DNA-dependent RNA polymerase used for in vitro transcription. Only SP6 DNA or DNA cloned downstream from an SP6 promoter can be used as a template for SP6 RNA Polymerase-directed RNA synthesis. The polymerase can incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates.

Supplier: Promega

The RiboMAX™ large scale RNA production systems consistently produce 2 to 5 mg/ml of RNA in a 1 ml reaction, about 10- to 20-fold more RNA than is produced with the standard Riboprobe® System transcription reaction.

Supplier: Promega

RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides.

Supplier: Promega

The pBR322 vector carries the genes for tetracycline and ampicillin resistance. pBR322 DNA digests typically and are used as molecular weight size markers in gel analysis of nucleic acids.

Supplier: Promega

Nucleic Acid Purification Kits and Reagents, Wizard® Midipreps DNA purification resin, 1000 ml

Supplier: Promega

Urea

Supplier: Promega

IPTG, Dioxane-Free (isopropyl-β-d-thiogalactopyranoside), is an inducer of β-galactosidase activity in many bacteria. Functioning as a lac analog, IPTG induces β-galactosidase activity by binding to and inhibiting the lac repressor. This product is used to differentiate recombinants from nonrecombinants in cloning strategies using vectors containing the lacZ or lacZ α-peptide gene..

Supplier: Promega

Tween® 20 (Polysorbate)

Supplier: Promega

Cell separation media, Ficoll® 400

Supplier: Promega

Polyethylene glycol 6,000

Supplier: Promega

BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity.

Supplier: Promega

Guanidinium thiocyanate

Supplier: Promega

High-quality deoxynucleotide triphosphates (dNTPs) are important for many key procedures such as cDNA synthesis, sequencing, cloning and genotyping.

Supplier: Promega

CATG G sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

These HpaII restriction enzyme recognises the cut site of C^˅CG G and G GC_˄C.