"PROVITRO"

Supplier: PROVITRO

The hMSC adipogenesis induction medium is a sterile liquid culture medium for inducing adipogenic differentiation of human mesenchymal stem cells (hMSC). The medium is delivered as a basal medium and is suitable for culturing hMSC after adding the supplement kit components.

Supplier: PROVITRO

Cancer Stem Cell Medium is a sterile liquid culture medium designed for the cultivation of human cancer stem cells. Delivered in the form of a basal medium, it becomes a complete and effective environment for the growth of human cancer stem cells upon the addition of supplement mix components.

Supplier: PROVITRO

The supplement kit designed for cultivating human smooth muscle cells (HSMC) provides an optimised formulation for promoting growth and maintaining the health of these cells. Upon adding the components of the supplement kit to a 500 ml basal medium, a specialised environment is created to support the culture of HSMCs. The formulation is specifically tailored for the initial seeding of 4000 cells per square centimeter, aiming to achieve confluence at approximately 90%. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. All components are subjected to rigorous biological assays. Each batch is specifically examined for the proliferating characteristics of human smooth muscle cells (HSMC). The cells cultured in the smooth muscle cell growth medium undergo thorough assessments, including examination of their morphology, adherence rate, colony-forming efficiency, and population doubling time.

Supplier: PROVITRO

The Provitro skeletal muscle cell growth medium is a sterile liquid culture medium for culturing human skeletal muscle cells (HSKMC). The medium is delivered as a basal medium and is suitable for culturing Provitro HSKMC after adding the supplement kit components. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROVITRO

The supplement kit for endothelial cell proliferation medium is suitable for culturing Provitro human endothelial cells after adding the supplement kit components to 500 ml basal medium The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. The supplement kit for endothelial cell priliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium, advanced is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROVITRO

Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the passage kit. After thawing store the passage kit in the dark at 2 °C to 8 °C for a maximum of 4 weeks. Take care: After thawing shelf time is limited to 4 weeks.
After thawing the passage kit can be stored in the dark at 2 °C to 8 °C for up to 1 month. Do not heat the kit over 37 °C or use uncontrollable sources of heat (e.g. microwave appliances).
Do not refreeze the kit. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.
Overtrypsination causes irreversible damage.
Because of the tryptic activity of this passage kit, do not exceed the recommended incubation period. Exposing the cells too long will cause irreversible damages to your culture.

Supplier: PROVITRO

Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.

Supplier: PROVITRO

In a serum-free cell culture system, it is very important to cryopreserve cells also in a serum-free freeze medium and not in a serum containing medium. Using an ordinary serum containing freeze medium, the serum affects the cell culture system over a long period. This influence can only be removed by dilution during a large number of passages after seeding.

Supplier: PROVITRO

These whole blood products are a versatile resource, offering a pure representation of peripheral blood that is ideal for a wide range of research and development endeavors. The whole blood is drawn from healthy donors, with various preservation options to meet specific research needs. Additionally, prospective collections of blood samples from individuals with specific medical conditions can be arranged upon request.

Supplier: PROVITRO

Our highly cell-concentrated Leukopak is a specialised product from leukapheresis using the Spectra Optia system. This advanced technology employs centrifugal mechanisms for continuous blood flow, extracting white blood cells from peripheral blood into a sterile bag with ACD-A anticoagulant. The enriched product includes monocytes, lymphocytes, platelets, plasma, and erythrocytes.

Supplier: PROVITRO

Human peripheral blood mononuclear cells (PBMCs) are utilised as source material for isolating lymphocytes (T cells, B cells and NK cells) and monocytes in research and across fields such as immunology, immuno-oncology, and cell and gene therapies.
All PBMCs are isolated through Ficoll density gradient separation from freshly obtained leukapheresis ACD-A packs and directly cryopreserved.

Supplier: PROVITRO

Human serum, the fluid component of blood obtained after the removal of fibrinogen and other clotting factors, serves numerous vital purposes. These include human cell culture, drug testing, tissue typing, and cell therapy research. Human blood serum does not contain white blood cells, red blood cells, or platelets. Human blood serum can be obtained from healthy donors in fresh or cryopreserved formats upon custom request.

Supplier: PROVITRO

Human plasma serves to suspend and transport red blood cells, white blood cells, and platelets throughout the body. It also contains essential components such as water, salts, enzymes, antibodies, and clotting factors like albumin and fibrinogen.

Supplier: PROVITRO

Isolated T cells, including CD3⁺ pan-T cells, CD4⁺ and CD8⁺ subgroups, have been obtained through immunomagnetic cell separation techniques employing positive or negative selection from mononuclear cells. These differentiated procedures ensure the precise isolation of the intended T-cell groups. CD4⁺ T cells play a role in coordinating immune responses, while CD8⁺ T cells exert a cytotoxic function.