530 Results for: "Merck Millipore (Novagen)"

Supplier: Merck Millipore (Novagen)

The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

Supplier: Merck Millipore (Novagen)

Insect GeneJuice® Transfection Reagent is formulated to maximise transfection efficiency of Sf9 insect cells. The reagent features extremely low toxicity to the cells and can be used for both transient and stable transfections in serum-containing or serum-free media, and for co-transfection of transfer plasmids with linearised virus DNA for the production of recombinant baculoviruses.

Supplier: Merck Millipore (Novagen)

Lysonase™ bioprocessing reagent is an optimised, ready-to-use convenient blend of rLysozyme™ solution and benzonase nuclease which is ideal for protein extraction.

Supplier: Merck Millipore (Novagen)

The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.

Supplier: Merck Millipore (Novagen)

BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.

Supplier: Merck Millipore (Novagen)

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

Supplier: Merck Millipore (Novagen)

PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.

Supplier: Merck Millipore (Novagen)

This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

Supplier: Merck Millipore (Novagen)

ColiRollers™ plating beads are a specially treated glass bead which offers a better way to perform plating bacteria for transformation and molecular cloning applications. ColiRollers™ beads eliminate the inconsistencies of conventional 'hockey stick' methods.

Supplier: Merck Millipore (Novagen)

Restriction grade thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases.

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Supplier: Merck Millipore (Novagen)

Overnight Express™ Autoinduction NMR Medium, 5 L kit

Supplier: Merck Millipore (Novagen)

The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

Supplier: Merck Millipore (Novagen)

The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

Supplier: Merck Millipore (Novagen)

Desoxyribonuclease I

Supplier: Merck Millipore (Novagen)

Prsf expression system 1 plus competen

Supplier: Merck Millipore (Novagen)

PELLET PAINT NF CO-PRECIPITANT

Supplier: Merck Millipore (Novagen)

PELLET PAINT NF CO-PRECIPITANT

Supplier: Merck Millipore (Novagen)

PELLET PAINT NF CO-PRECIPITANT

Supplier: Merck Millipore (Novagen)

5% Alkali-Soluble Casein 1 * 225 ml

Supplier: Merck Millipore (Novagen)

3 DNA KIT BACMAGIC 1 * 1 KIT

Supplier: Merck Millipore (Novagen)

Oligo(Dt) Primer 1 * 20 µG

Supplier: Merck Millipore (Novagen)

Pet-31B(+) Dna Alwn I Digest* 1 * 40 Tests

Supplier: Merck Millipore (Novagen)

Pt7Blue Blunt Kit 1 * 40 Tests

Supplier: Merck Millipore (Novagen)

Pt7Blue-3 Blunt Kit 1 * 40 Tests

Supplier: Merck Millipore (Novagen)

Pstblue-1 Blunt Vector 1 * 40 Tests

Supplier: Merck Millipore (Novagen)

Pstblue-1 Blunt Kit 1 * 40 Tests

Supplier: Merck Millipore (Novagen)

Pbac-6 Transfer Plasmid 1 * 10 µG

Supplier: Merck Millipore (Novagen)

5x antigenplus buffer (ph 10) 1 * 1 items