28228 Results for: "Biotium"

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

Supplier: Biotium

Stains specifically designed for DNA in polyacrylamide gels.

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.

Supplier: Biotium

One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.

Supplier: Biotium

GelRed and GelGreen are highly sensitive DNA/RNA gel stains made safe by preventing penetration into cells.

Supplier: Biotium

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

Supplier: Biotium

Viability PCR With PMA and PMAxx™ dyes

Supplier: Biotium

The Super HT PAP pen draws a water repellent circle around slide mounted tissue and prevents the waste of valuable reagents by keeping the liquid pooled in a single droplet. The regular sized super HT PAP pen can be used in about 800 applications, while the mini sized super HT PAP Pen will last about 400 applications.

Supplier: Biotium

Thiazole green, which is structurally identical to SYBR® green I nucleic acid gel stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

Supplier: Biotium

PMAxx™ dye is a DNA modifier used for viability PCR (v-PCR). V-PCR is a powerful PCR-based, culture-independent, method for rapid and sensitive quantification of microbial viability.

Supplier: Biotium

Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.

Supplier: Biotium

PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

Supplier: Biotium

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

Supplier: Biotium

PE, disposable, sterile. Mini cell scrapers for harvesting cells or cell lysates from microwell plates.

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Supplier: Biotium

TrueBlack™ is a reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a cell and tissue types with age.

Supplier: Biotium

PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.

Supplier: Biotium

Paraformaldehyde, 4% in PBS is a ready-to-use fixation solution for cells or tissues.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: Biotium

Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions.

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Supplier: Biotium

6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

Supplier: Biotium

Anti-IgG Goat Polyclonal Antibody (CF™ 647)

Supplier: Biotium

Anti-IgG Donkey Polyclonal Antibody (CF™ 633)

Supplier: Biotium

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations.

Supplier: Biotium

Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.

Supplier: Biotium

Anti-IgG Goat Polyclonal Antibody (CF™ 350)