Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Screen Quest™ 10X Calcium Assay Buffer with Phenol Red Plus™
Supplier: AAT Bioquest
Ready-to-use buffer that is optimised for calcium assays.
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PhosphoWorks™ Colorimetric Phosphate Assay Kit, Blue Color
Supplier: AAT Bioquest
Phosphate is involved in many biological reactions.
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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*
Supplier: AAT Bioquest
Caspases play important roles in apoptosis and cell signaling.
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Native Streptavidin (from Streptomyces avidinii), iFluor® 568
Supplier: AAT Bioquest
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.
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iFluor® 594, Maleimide
Supplier: AAT Bioquest
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies.
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iFluor® 555, Alkyne
Supplier: AAT Bioquest
AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies.
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Violet 510 acid, mFluor™ fluorescent probe
Supplier: AAT Bioquest
AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications.
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ICG-PEG12-OSu ≥90%
Supplier: AAT Bioquest
Indocyanine green (ICG) is a cyanine dye used in medical diagnostics.
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Fura 2-AM calcium indicator
Supplier: AAT Bioquest
Calcium measurement is critical for numerous biological investigations.
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PE-Cy5.5 Tandem, ReadiUse™ fluorescent probe, preactivated
Supplier: AAT Bioquest
PE-Cy5.5 is a popular color used in flow cytometry.
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Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*
Supplier: AAT Bioquest
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Fluorimetric Glucose Quantitation Kit
Supplier: AAT Bioquest
Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Native Megathura crenulata KLH (from Megathura crenulata), Chloramphenicol
Supplier: AAT Bioquest
Chloramphenicol-KLH conjugate is used for developing anti-chloramphenicol antibodies that are typically used in an ELISA assay.
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Native Armoracia rusticana HRP (from Armoracia rusticana), 3-Amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)
Supplier: AAT Bioquest
3-Amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)-HRP conjugate is used for developing an ELISA assay for quantifying 3-Amino-5-morpholinomethyl-2-oxazolidinone (AMOZ).
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Native Bovine Bovine Serum Albumin (from Serum), Ofloxacin
Supplier: AAT Bioquest
Ofloxacin-BSA Conjugate is used for developing anti-ofloxacin antibodies that are typically used in an ELISA assay.
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Native Megathura crenulata Keyhole limpet hemocyanin (from Serum), Cinoxacin
Supplier: AAT Bioquest
Cinoxacin-KLH Conjugate is used for developing anti-cinoxacin antibodies that are typically used in an ELISA assay.
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Native Horseradish Horseradish peroxidase (from Serum), Sulfadimidine
Supplier: AAT Bioquest
Sulfadimidine-HRP conjugate is used for developing an ELISA assay for quantifying sulfadimidine with an ELISA assay.
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Native Bovine Bovine Serum Albumin (from Serum), Spectinomycin
Supplier: AAT Bioquest
Spectinomycin-BSA conjugate is used for developing anti-spectinomycin antibodies that are typically used in an ELISA assay.
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Fluorescein dioctanoate (Fluorescein dicaprylate) ≥95%
Supplier: AAT Bioquest
Fluorescein dicaprylate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dicaprylate group producing the highly fluorescent product fluorescein.
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5(6)-Carboxy-2′,7′- dichlorofluorescein diacetate mixture of isomers ≥95%
Supplier: AAT Bioquest
It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.
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1,1-Didodecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate [DiIC12(3) perchlorate] ≥95%
Supplier: AAT Bioquest
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures.
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3,3-Diheptyloxacarbocyanine iodide [DiOC7(3) iodide] ≥95%
Supplier: AAT Bioquest
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures.
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1,1-Diododecyl-3,3,3,3-tetramethylindodicarbocyanine-5,5-diSulphonic acid [DiIC12(5)-DS] ≥95%
Supplier: AAT Bioquest
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures.
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DiIC1(5) iodide ≥95%
Supplier: AAT Bioquest
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures.
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PhosphoWorks™ Luminometric ATP Assay Kit, DTT-free
Supplier: AAT Bioquest
Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling.
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PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Enhanced Selectivity
Supplier: AAT Bioquest
Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.
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FluoroQuest™ Fluorescence Signal Enhancing Solution (5 ml)
Supplier: AAT Bioquest
When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50 to 70%.
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Pluronic® F-127 Cell Culture Tested
Supplier: AAT Bioquest
Pluronic® F-127 is a nonionic surfactant that is 100% active and relatively non-toxic to cells, and frequently used with dye AM esters to improve their water solubility.
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Annexin V-iFluor® 555 Conjugate
Supplier: AAT Bioquest
Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.