T7 RNA polymerase

T7 RNA Polymerase is a DNA-dependent RNA polymerase specific for T7 promoter sequences, that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis.

• >90% pure as determined by SDS polyacrylamide gel electrophoresis
• Free of detectable levels of contaminating RNase and DNase activity (<1% release)
• Incorporates ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates
• Source: Recombinant E. coli strain

Applications:
Generation of templates for in vitro translation
Generation of probes for nucleic acid hybridizations
Generation of RNA processing substrates
Generation of antisense RNA

Storage buffer: 20 mM potassium phosphate (pH 7,7 at 25 °C), 1 mM EDTA, 10 mM DTT, 100 mM NaCI, 0,1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5 nmol of CTP into acid-insoluble product in 60 minutes at 37 °C in a total volume of 100 μl.

Delivery information: Supplied with DTT (100 mM) and transcription optimized 5X buffer: 200 mM Tris-HCl (pH 7,9 at 25 °C), 30 mM MgCl₂, 10 mM spermidine, 50 mM NaCl.