The ReliaPrep RNA miniprep systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes from very small amounts of input material.

• Efficiently purify RNA from as few as 100 cells or 0,25 mg tissue
• Simple, 30-minute protocol
• Elutes in small volume, generating highly concentrated RNA
• No phenol/chloroform or ß-mercaptoethanol required

The ReliaPrep RNA cell miniprep system provides a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15 µl). Using this membrane-based purification system, from 100 to 5×10⁶ cultured cells or 0,25 to 20 mg of tissue can be processed per purification.

The system incorporates a DNase treatment step directly on the minicolumn membrane and effectively removes substances that can inhibit downstream assays. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, resulting in pure RNA that does not require additional purification or concentration of the RNA for use in demanding applications.