Fluorogenic substrate for several matrix metalloproteinases (MMPs).

• MMPs cleave this peptide between Gly-Leu

It is cleaved by MMP-1 (kcat/Km=3,3×10M-1s-1), MMP-2 (kcat/Km=5,8×10), MMP-3 (kcat/Km=2,2×10), MMP-7 (kcat/Km=1,2×10), MMP-8, MMP-9 (kcat/Km=6,9×10), and MMP-26 (kcat/Km=4,9×10) Ex/Em=280 nm/346 nm, although 283/350,360 are also appropriate.

Fluorescence at 346 nm by tryptophan occurs once cleavage separates it from the quenching aromatic Dnp moiety.