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Human recombinant MGMT (from E. coli)
Human recombinant MGMT (from E. coli)
Catalog # PRSI92-212
Supplier:  ProSci Inc.
CAS Number:  
Human recombinant MGMT (from E. coli)
Catalog # PRSI92-212
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Protein/peptide type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Tag sequence:
    N-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
  • Protein synonyms:
    O-6-methylguanine-DNA-alkyltransferase|6-O-methylguanine-DNAmethyltransferase|MGMT|Methylated-DNA--protein-cysteine methyltransferase
  • Protein/peptide name:
    MGMT
  • Purity:
    > 95% as determined by reducing SDS-PAGE
  • Molecular weight:
    23.8 kD
  • Sequence:
    Met1-Asn207
  • Formulation:
    Supplied as a 0.2 um filtered solution of 20mMTrisHCl,1mM EDTA, pH 8.0 . Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

Specifications

About this item

MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyses the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.

Fusion-Tag: N-6 His tag

This recombinant protein can be used for biological assays. For research use only.