Human recombinant MGMT (from E. coli)
Catalog # PRSI92-212
Supplier: ProSci Inc.
CAS Number:
Specifications
- Pk:50 µG
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:N-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
- Protein synonyms:O-6-methylguanine-DNA-alkyltransferase|6-O-methylguanine-DNAmethyltransferase|MGMT|Methylated-DNA--protein-cysteine methyltransferase
- Protein/peptide name:MGMT
- Purity:> 95% as determined by reducing SDS-PAGE
- Molecular weight:23.8 kD
- Sequence:Met1-Asn207
- Formulation:Supplied as a 0.2 um filtered solution of 20mMTrisHCl,1mM EDTA, pH 8.0 . Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
Specifications
About this item
MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyses the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.
Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays. For research use only.