ProTEV Plus is an improved 48 kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity.

• Engineered protease with greater stability
• Prolonged activity over native TEV protease
• Active over broad pH (5,5 to 8,5) and temperature (4 to 30 °C) range
• Use with in-solution and on-column digestions
• Convenient removal of ProTEV Plus using Ni-based affinity resins after cleavage (HQ-Tagged)
• Easy to use in multiple experimental formats

It is a highly specific proteolytic enzyme that cleaves within a seven-amino-acid sequence (ENLYFQ(G/S)). It can be used to cleave protein fusions that have been engineered with the above amino acid sequence at the desired cleavage site

Suitable for cleavage of affinity tags from fusion proteins after protein purification and removal of HaloTag® protein during protein purification or expression of fused proteins of interest.