PDE-Glo™ Phosphodiesterase Assay is a luminescent, high-throughput screening (HTS) method for measuring cyclic nucleotide phosphodiesterase (PDE) activity from purified sources. The PDE-Glo™ Assay is used for screening library compounds to identify inhibitors of either cAMP- or cGMP-specific PDEs. It is optimised to work with both cAMP- and cGMP-dependent phosphodiesterases.

• Sensitive and easy to use
• Assay complete in >1 hour after PDE reaction
• Scalable to 1536-well plate formats
• Simple luminescence detection

Cyclic nucleotide phosphodiesterases are involved in many cellular processes due to their ability to hydrolyse, and thus control, the levels of the second-messenger signaling molecules cAMP and cGMP. The availability of selective inhibitors for PDEs has facilitated their use as tools to study cyclic nucleotide signaling and paved the way to investigate the role of PDEs in cellular and tissue pathologies.