Cell signaling assays, ProFluor® Ser/Thr PPase Assay
Supplier: Promega
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About this item
ProFluor® Ser/Thr PPase Assay measures purified serine/threonine protein phosphatase activity in a multiwell plate format and involves 'add-mix-read' steps only. The assay works with protein phosphatase 1 (PP1), PP2A, PP2B and PP2C.
- Ideal for high-throughput phosphatase assays in 96- or 384-well plate formats
- Minimal test compound interference
- Control peptide included (AAF-AMC) for monitoring protease activity, reducing false-positive hits
- No radioactive waste disposal and safety issues
ProFluor® Ser/Thr PPase Assay produces excellent Z´ values (>0,8) in either 96- or 384-well plate formats and easily distinguishes known phosphatase inhibitors from other compounds.
Delivery: Kit includes stabiliser reagent, protease reagent, S/T PPase R110 substrate, control AMC substrate, MgCl₂, MnCl₂, CaCl₂, NiCl₂, Reaction buffer B (5X), Termination buffer B (5X).
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