PolyATtract® mRNA Isolation Systems use MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA.

• Protocol complete in approximately 45 minutes
• Isolated mRNA is suitable for use in in vitro translation, RT-PCR and cDNA synthesis

The systems use a biotinylated oligo(dT) primer to hybridise, at high efficiency in solution, to the 3' poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The highly pure mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionised water.

The PolyATtract® mRNA Isolation Systems are available in two basic configurations, which depend on the amount of input RNA:
PolyATtract® Systems I and II are designed for larger amounts of input RNA; either system provides reagents sufficient for three isolations, each from 1 to 5 mg of total RNA. PolyATtract® System I does not include a magnetic separation stand. PolyATtract® System II includes the stand.
PolyATtract® Systems III and IV provide the MagneSphere® Streptavidin Paramagnetic Particles in smaller aliquots, suitable for use with smaller amounts of input RNA. Each of these systems contains reagents sufficient to perform fifteen mRNA isolations, starting with 1 mg or less total RNA per isolation.