ProteaseMAX™ Surfactant ensures fast and efficient protein digestion with proteases such as trypsin, chymotrypsin and Lys-C.

• Enhances enzymatic performance of trypsin, chymotrypsin and Lys-C
• Saves time and labour when used with in-gel protein digestion – complete digestion in 1 hour

For in-gel protein digestion, ProteaseMAX™ Surfactant offers time and labor savings. Digestion step is complete in 1 hour, and the surfactant provides concurrent extraction of peptides from gels, eliminating the need for post-digestion peptide extraction. The surfactant also improves recovery of longer peptides that are retained in the gel under a standard extraction protocol.

For in-solution digestions, ProteaseMAX™ Surfactant solubilises proteins, including difficult proteins (i.e., membrane proteins), and enhances protein digestion by providing a denaturing environment prior to protease addition.

ProteaseMAX™ Surfactant degrades over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). No long-term negative effect of the residual surfactant on the ion optics and capillary of mass spectrometers has been observed. ProteaseMAX™ Surfactant can be used with existing in-gel or in-solution digestion protocols.