Large scale RNA production system, T7 RiboMAX™ Express
Catalog # PROMP1320
Supplier: Promega
CAS Number:
Specifications
- Pk:50 Tests
- Description:Large scale RNA production system, T7 RiboMAX™ Express
Specifications
About this item
The T7 RiboMAX™ Express large scale RNA Production system is an in vitro transcription system designed for the consistent production of milligram amounts of RNA in a short amount of time.
- Consistent production of milligram amounts of RNA
- Minimal pipetting steps and setup time
- Efficiently transcribes DNA templates of varying sizes – works with transcripts as short as 21 bp
- Yields of 5 to 8,5 mg/ml are generated in 30 minutes, compared to 2 to 4 hours with other commercially available systems
- To minimise pipetting steps and errors, the 2X Transcription Buffer includes all four rNTPs
- Includes RQ1 RNase-Free DNase for the removal of plasmid template after transcription
Applications:
Generation of large quantities of uncapped transcripts for in vitro or in vivo translation.
In vitro synthesis of tRNA, rRNA, RNA virus genomes and ribozymes.
Production of substrates for RNA splicing, RNA secondary structure, antisense RNA and RNA:protein interaction studies.
Synthesis of long RNAs for non mammalian RNA interference.
Delivery: Kit includes RiboMAX™ Express T7 2X Buffer, RQ1 RNase-free DNase, T7 Express Enzyme Mix, sodium acetate 3,0 M (pH 5,2), pGEM® Express positive control template and nuclease-free water.
Caution: J