The Apo-ONE® Homogeneous Caspase-3/7 Assay provides the necessary reagents for fast and sensitive measurement of active caspase-3 and -7 in a homogeneous format.

• Simple 'add-mix-measure' format combined with the high sensitivity of the assay dramatically decreases the 'time to first result' by eliminating cumbersome sample preparation and lengthy incubation steps
• Optimised caspase-3/7 activity buffer, in conjuncton with the R110-labeled substrate, allows for increased sensitivity over existing fluorescent caspase assay methods
• Flexible assay design (from cuvette to 384-well plate) allows for adaptable formats and throughput needs

The assay includes a profluorescent caspase-3/7 consensus substrate, rhodamine 110 bis-(N-CBZ-l-aspartyl-l-glutamyl-l-valyl-aspartic acid amide) (Z-DEVD-R110), and an optimised bifunctional cell lysis/activity buffer. The buffer efficiently lyses cultured mammalian cells and supports optimal caspase-3/7 enzymatic activity. The substrate and buffer are combined to make the Apo-ONE® Caspase-3/7 Reagent that is added directly to samples. Upon cleavage on the C-terminal side of the aspartate residue in the DEVD peptide substrate sequence by caspase-3/7 enzymes, the rhodamine 110 becomes fluorescent when excited at a wavelength of 498 nm. The emission maximum is 521 nm. The amount of fluorescent product generated is representative of the amount of active caspase-3/7 present in the sample.