rLys-C, MS grade, is a recombinant Lys-C expressed in E. coli. It cleaves at C-terminus of lysines.

• Sequence origin of rLys-C is Protease IV from Pseudomonas aeruginosa
• Optimal activity in the pH range of 8 to 9
• Retains activity in denaturing conditions such as 8 M urea
• Available lyophilised or frozen

The protease is supplied in a lyophilised form along with a reconstitution buffer, which is formulated to increase stability of rLys-C solution. It has been shown to be free of contaminating peptides by reverse-phase HPLC.

This rLys-C is used for digestion of single proteins and complex protein mixtures in-solution and in-gel. It is also applicable for peptide mapping and protein identification.

Frozen rLys-C solution can be stored for a month at −20 °C without detectable loss of activity.