The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.

• Measures physiologically relevant GSH/GSSG ratios
• Simple protocol minimises sample handling, reducing variability

The assay generates values for total glutathione and GSSG. Since the value for total glutathione represents the signals from both the reduced and oxidised glutathione, subtracting the GSSG reaction signal from the total glutathione signal yields the value of reduced glutathione in the sample.