The Kinase-Glo® Max Luminescent Kinase Assays are homogeneous non-radioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. The Kinase-Glo® Max Assay is linear to 500 μm ATP, making it well suited for use with kinases with high Km for ATP, as well as for screening for kinase inhibitors that do not compete at the ATP binding site.

• Simple
• Scalable
• Easy to automate

Kinase-Glo® Max Luminescent Kinase Assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS), and they can be used to assay protein, lipid and sugar kinases. The assay procedure involves addition of a single reagent directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity.