FluoroTect™ GreenLys in vitro Translation Labelling System enables fluorescent labelling and detection of in vitro synthesised proteins, based on a lysine-charged tRNA labelled with the fluorophore BODIPY®-FL at the ε position. Fluorescent lysine residues are incorporated into synthesised proteins during in vitro translation reactions, eliminating the need for radioactivity.

• Data in minutes versus overnight exposures associated with radioactivity-based systems
• In-gel detection, no need to transfer, fix or dry gels
• No radioactivity required

Detection of the labelled proteins is accomplished in 2 to 5 minutes directly 'in-gel' by use of a laser-based fluorescent gel scanner. This eliminates any need for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively-labelled amino acids. The convenience of 'in-gel' detection also avoids the time-consuming electroblotting and detection steps of conventional non-isotopic systems.