Rabbit Reticulocyte Lysate, Untreated contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA.

• Contains tRNA, ribosomes, amino acids, initiation, elongation and termination factors needed for protein synthesis
• No micrococcal nuclease treatment
• Consistent reticulocyte production from every lot

Applications: Isolation of the cellular components necessary for protein synthesis; Translation of exogenous mRNA, after further optimisation, including the addition of energy-regenerating systems, hemin, endogenous tRNA and micrococcal nuclease to remove endogenous mRNA.