The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or λ vectors containing a T7 promoter by providing an extract that contains T7 RNA polymerase for transcription and all components needed for translation. The investigator only supplies cloned DNA containing a T7 promoter and a ribosome binding site.

• Can translate using any clone that has a T7 promoter and a ribosome binding site - other S30 extracts require an E. coli promoter
• Stable: Reduced chance of expressed proteins degrading
• Complete: Contains all necessary components for coupled transcription/translation
• Low background: System synthesises very low levels of endogenous proteins
• Premix is optimised for each lot of S30 Extract and contains all other required components (except amino acids), such as ribonucleotides, tRNAs, PEP (phosphoenol pyruvate) and salts

This product is prepared by modifications of the method described by Zubay from an E. coli strain B deficient in OmpT endoproteinase and ion protease activity. This results in greater stability of expressed proteins that would otherwise be degraded by proteases if expressed in vivo.

Storage: Store extract at –70 °C.