The Proteasome-Glo™ 3-Substrate System consists of three homogeneous bioluminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. Each of these three assays is also available separately.

• Simple 'add-mix-measure' protocol
• Results in 10 to 30 minutes after reagent addition
• Luminescent assay avoids inherent fluorescent background signals, providing excellent signal-to-background ratio

The luminogenic substrates provided for the chymotrypsin-like, trypsin-like and caspase-like activities are Suc-LLVY-aminoluciferin, Z-LRR-aminoluciferin and Z-nLPnLDaminoluciferin, respectively. Each substrate is added to a buffer system optimised for proteasome activity and luciferase activity to make a Proteasome-Glo™ Reagent for a particular catalytic activity. The individual Proteasome-Glo™ Reagent is added to test samples in an 'add-mix-measure' format, resulting in proteasome-induced cleavage of the particular substrate. Substrate cleavage generates a 'glow-type' luminescent signal produced by the luciferase reaction. In this homogeneous coupled-enzyme format, the signal is proportional to the amount of proteasome activity.