High yield protein expression system, S30 T7
: Promega
S30 T7 high yield protein expression system can be used to express up to 500 μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.
- Flexible detection of expressed proteins by staining or fluorescence or biotinylated labels
- Greater stability and enhanced expression of target proteins.
The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity.
Suitable for protein:protein interactions, protein:DNA interactions, enzyme activity and antibody production.
: Includes 3×200 µl S30 Pre-mix Plus, 1×20 µl S30 T7 control DNA, 3×150 µl T7/S30 extract for circular DNA and 1×1250 µl nuclease-free water.
- Storage temperature:–70 °C
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