Extract system for linear templates, E. coli S30
PROML10300450
: Promega
:
- Pk:450 µl
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- Description:E. coli S30 Extract System for Linear Templates, 30 reactions
- Environmentally Preferable:
- Storage conditions:Store all components at –70 °C. Avoid prolonged
exposure to CO2 and multiple freeze-thaw cycles, which may have an adverse effect on the activity/performance.
The E. coli S30 Extract System for Linear Templates allows successful transcription/translation of linear DNA templates. The investigator need only provide linear DNA containing a prokaryotic E. coli-like promoter (such as lacUV5, tac, λPL (con) and λ-PR). A ribosome binding site is required to direct the synthesis of proteins in vitro.
- Many templates can be used: DNA fragments, PCR-synthesised DNA, ligated overlapping oligonucleotides, in vitro-generated RNA and
- prokaryotic RNA
- Stable: Reduced chance of expressed proteins degrading
- Complete: Contains all necessary components for coupled transcription/translation
- Low background: System synthesises very low levels of endogenous proteins
- Premix is optimised for each lot of S30 Extract and contains all other required components (except amino acids), such as ribonucleotides, tRNAs, PEP (phosphoenol pyruvate) and salts
In vitro generated RNA from DNA templates lacking an E. coli promoter may also be used in this system, but protein yields will be decreased to 1 to 10% of that produced from linear DNA templates.
Storage: Store at –70°C.
: Amino acid mxture minus cysteine, S30 extract, linear, pBESTluc™ DNA, linear (control), luciferase assay reagent, luciferase dilution reagent, S30 premix without amino acids, amino acid mixture minus leucine, amino acid mixture minus methionine.