High performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94 to 95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 °C for convenient, room-temperature reaction set-up.

• Eliminate non specific amplification with a hot-start enzyme
• Detect lower-copy-number targets
• Optimise reaction conditions with a Flexi Reaction Buffer and separate MgCl₂
• Supplied at a concentration of 5 U/μl

Hot-start PCR is advantageous for some amplification targets because it may eliminate or minimise primer-dimer and non specific products. In some cases, hot-start PCR may improve yields. GoTaq® G2 Hot Start Polymerase exhibits 5 →3 exonuclease activity.