CellTiter-Glo® One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells.

• Ready-to-use assay eliminates several protocol steps
• Designed for use with multiwell-plate formats, for automated high throughput screening
• No reagent preparation is required
• Data can be recorded in 10 minutes or less once after the addition of reagent

The homogeneous 'add-mix-measure' format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The assay generates a stable 'glow-type' luminescent signal with a half-life of greater than three hours. This extended half-life eliminates the need for reagent injectors and provides flexibility for continuous or batch-mode processing of multiple plates.

100 ml of One Solution Assay is sufficient for 1000 assays at 100 µl/assay in 96-plates or 4000 assays at 25 µl/assay in 384-well plates. 500 ml of One Solution Assay is sufficient for 5000 assays at 100 µl/assay or 20000 assays at 25 µl/assay in 384-well plates.

The CellTiter-Glo Assay is designed for use with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) in 96 to 1536-well format and cell proliferation and cytotoxicity assays.