The BacTiter-Glo™ Microbial Cell Viability Assay is used for determining the number of viable microbial cells in culture based on quantitation of the ATP present. This homogeneous assay involves adding a single reagent, BacTiter-Glo™ Reagent, directly to bacterial cells cultured in medium and measuring luminescence.

• Reduces pipetting errors
• Data can be recorded in 5 minutes or less once after the addition of reagent
• Assay can be used to detect a variety of bacteria and fungi

The formulation of the reagent supports bacterial cell lysis and generation of a luminescent signal. The luminescent signal is proportional to the amount of ATP present, which is directly proportional to the number of viable cells in culture. The assay relies on the properties of a thermostable luciferase (Ultra-Glo™ Recombinant Luciferase) and a buffer formulation for extracting ATP from bacteria.