Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterise their protein products and investigate transcriptional and translational control.

• Optimised for translation and ready to use
• Contains an energy-regenerating system, a mix of tRNAs, hemin, potassium chloride and magnesium acetate

Rabbit Reticulocyte Lysate System, Nuclease Treated is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis.

Applications: In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein; characterisation of mRNA products; co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction.