The MultiTox-Glo Multiplex Cytotoxicity Assay is a sequential-reagent-addition fluorescent and luminescent assay that measures the relative number of live and dead cells in cell populations.

• Normalise data with a built-in internal control
• Immediately identify more false-positives and false-negatives

The assay gives ratiometric, inversely correlated measures of cell viability and cytotoxicity, which correlate with established methods for measuring viability and cytotoxicity. The ratio of viable cells to dead cells is independent of cell number and, therefore, can be used to normalise data. The complementary cell viability and cytotoxicity measures reduces errors associated with pipetting and cell clumping, as well as serves as an internal control to allow identification of errors resulting from chemical interference from test compounds or media components. A luminescent readout allows for a reduction in the statistical probability of false-positives (or false-negatives) and eliminates fluorescence interference issues.