Kinase assay, ADP-Glo™

The ADP-Glo™ kinase assay measures ADP formed from a kinase reaction. The ADP is converted into ATP, which is used to generate light in a luciferase reaction. The luminescence generated correlates with kinase activity.

Use with virtually any kinase
Stable luminescent signal
Sensitive to low concentrations of ADP

The assay is well suited for measuring the effects of chemical compounds on the activity of many purified kinases, making it ideal for primary screening as well as kinase selectivity profiling.

ADP-Glo kinase assays contain enough reagent to perform the indicated number of reactions in 384-well format using 5 μl, 5 μl, and 10 μl of a kinase reaction; ADP-Glo Reagent; and kinase detection reagent, respectively, per sample. The system also can be used in 96-well plates using 25µl:25µl:50µl for a total of 80 assays.

The assay measures the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening and kinase selectivity profiling. The ADP-Glo Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1 mM ATP.

Storage conditions:
Store the system at –20 °C. Before use, thaw all reagents completely at room temperature. Once thawed, components should be thoroughly mixed before use. Once prepared, the Kinase Detection Reagent (Kinase Detection Buffer and Substrate) should be divided into aliquots and stored at –20 °C.
Storage temperature:
–30...–10 °C