CYP3A4 (luciferin-PPXE) DMSO tolerant assay and screening systems, P450-Glo™

Supplier: Promega

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Supplier: Promega

About this item

The P450-Glo CYP3A4 with luciferin-PPXE assays provide a homogeneous, luminescent method for measuring cytochrome CYP3A4 activity in hepatocytes.

Broad dynamic range, low background and better sensitivity for less ambiguous data
Luminescent output eliminates interference from fluorescent test compounds
Optimal data quality for microplate reader assay
Simplified, homogeneous experimental protocol

The P450-Glo CYP3A4 with luciferin-PPXE screening system provides a complete set of reagents for performing luminescent cytochrome P450 assays. The membranes are prepared from baculovirus-infected insect cells and contain human cytochrome P450, P450 reductase, and cytochrome b5. It also contains a membrane fraction devoid of cytochrome P450 activity as a negative control. The assays are ideal for testing the effects of drugs and new chemical entities on cytochrome P450 enzyme activities.

Delivery: Assay system contains Luciferin-PPXE, 50 mM (1×15 or 2×15 µl); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer (1×10 or 1×50 ml). Screening system contains P450-Glo CYP3A4 assay includes Luciferin-PPXE, 50 mM (2×15 µl); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer (50 ml) and human CYP3A4 enzyme system includes CYP3A4 (1 pmol/µl), reductase and b5 (500 µl); control membranes (100 µl); potassium phosphate buffer, 1M (pH 7.4) (2×5 ml); luciferin-free water (50 ml); NADPH regeneration system solution A (2,75 ml); NADPH regeneration system solution B (0,6 ml).