CYP1A2 assay and screening systems, P450-Glo™

Supplier: Promega

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Supplier: Promega

About this item

The P450-Glo CYP1A2 assays provide a homogeneous, luminescent method for measuring cytochrome CYP1A2 activity.

Broad dynamic range, low background and better sensitivity for less ambiguous data
Luminescent output eliminates interference from fluorescent test compounds
Optimal data quality for microplate reader assay
Simplified, homogeneous experimental protocol

The P450-Glo CYP1A2 screening system provides a complete set of reagents for performing luminescent cytochrome P450 assays. The membranes are prepared from baculovirus-infected insect cells and contain human cytochrome P450, P450 reductase, and cytochrome b5. It also contains a membrane fraction devoid of cytochrome P450 activity as a negative control. The assays are ideal for testing the effects of drugs and new chemical entities on cytochrome P450 enzyme activities.

Delivery: Assay system contains Luciferin-ME, 5 mM (1×200 µl or 1×1 ml); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer (1×10 or 1×50 ml). Screening system contains P450-Glo CYP1A2 assay includes Luciferin-ME, 5 mM (1 ml); luciferin detection reagent (lyophilised) (1 vial); reconstitution buffer (50 ml) and human CYP1A2 enzyme system includes CYP1A2 (1 pmol/µl) and reductase (500 µl); control membranes (100 µl); potassium phosphate buffer, 1M (pH 7.4) (5 ml); luciferin-free water (50 ml); NADPH regeneration system solution A (2,75 ml); NADPH regeneration system solution B (0,6 ml).