The Caspase-Glo® 3/7 assay is a homogeneous luminescent assay that measures caspase-3/7 activities. The assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimised for caspase-3/7 activity, luciferase activity and cell lysis. This liberates free aminoluciferin, which is consumed by the luciferase, generating a 'glow-type' luminescent signal. The signal is proportional to caspase-3/7 activity. It is designed for use with multiwell plate formats using either purified enzyme or cells in culture.

• Add-mix-measure protocol makes the assay easy to automate
• Low background luminescence results in excellent signal-to-noise ratios, allows assay to be performed in 96- or 384-well formats
• No sample preparation, manipulation and extended incubation times required, maximum sensitivity can be achieved in as little as 15 minutes to 1 hour
• Delivers excellent Z´ factor values in cell and purified enzyme models
• Extended-glow signal allows the plates to be read over a 3 hour period of time for batch processing and no injectors required