CellTiter 96® Non-radioactive Cell Proliferation Assay is a collection of qualified reagents that provide a convenient method of determining viable cell number. The CellTiter 96® Assay is a modification of the MTT assay method described by Mosmann and incorporates several improvements to the method that address previous technical problems, including serum protein precipitation caused by adding organic solvent, interference by phenol red, incomplete solubilisation of the formazan crystals resulting in lower sensitivity, and stability of the coloured product.

• Detect as few as 1000 cells/well with a 96-well plate reader – greater sensitivity than the neutral red assay procedure
• Use with a variety of cells: Assay mammalian, plant and yeast cells
• Requires no scintillation cocktail or radioactive waste disposal
• Perform the assay in a 96-well plate with no washing steps, no cell harvesting and no scintillation counting
• Requires no weighing or mixing of dye compounds and follows either a 4-hour or overnight protocol