The PureYield™ plasmid Midiprep system is designed to isolate transfection quality plasmid DNA. The system provides a rapid method for purification of 100 to 200 μg of plasmid DNA from 50 ml bacteria culture. An alternative protocol allows purification of over 400 μg of high copy number plasmid from 250 ml of E. coli culture.

• Improved productivity: Vacuum protocol allows plasmid DNA purification in as little as 30 minutes
• High purity and concentration of plasmid DNA gives proven performance in transfection, in vitro expression and other molecular biology applications
• Simple protocol eliminates tedious high speed centrifugation, gravity drip columns, and post elution alcohol precipitation
• PureYield™ membrane column allows purification of large amounts of plasmid DNA, exceeding the capabilities of other midiprep systems

The system incorporates a unique endotoxin removal wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation such as TnT® Quick Coupled transcription/translation system. Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.