The GoTaq® 2-Step RT-qPCR system facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components such as GoScript™ reverse transcription system and GoTaq® qPCR master mix. It includes an optimised reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

• Sensitive detection for earlier quantitation of low and high copy number targets over a broad dynamic range
• Resistant to a wide range of PCR inhibitors
• Compatible with both fast and standard cycling methods on most Real-Time PCR instruments
• Exceptional room temperature set-up is easy for automated and high throughput detection