The ImProm-II™ reverse transcription system produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimised reaction buffer and ImProm-II™ Reverse Transcriptase provided in the system together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1 to 20 μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.

• Amenable to full-length RT-PCR: reverse transcribe long RNA templates up to 8,9 Kb
• Microarray compatible: may be used to incorporate regular, Cy®3-modified, Cy®5-modified and amino-allyl-modified nucleotides
• Easy to use: system provides all reagents necessary for efficient reverse transcription
• Scalable and flexible: 1 to 20 μl of the initial reverse transcription reaction may be used in subsequent PCR, and the optimised buffer allows coupled RT-PCR
• Versatile: Can be used with thermostable DNA polymerase of choice