Genomic DNA purification system, Wizard® SV
: Promega
The Wizard® SV genomic DNA purification system provides a fast, simple, membrane based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K. Amplifiable genomic DNA can be isolated from up to 5×10⁶ cells, 20 mg of tissue or up to 1,2 cm of a mouse tail tip without a centrifugation clearing step.
- Genomic DNA can be obtained approximately 20 minutes after lysis
- High yield – purify 20 to 30 μg of DNA per prep from 1,2 cm mouse tail
: The components in the kit can be ordered separately.
: The complete kit includes Wizard® SV minicolumns, collection tubes, nuclei lysis solution, RNase A solution, column wash solution (CWA), nuclease free water, EDTA (0,5 M) pH 8,0, and Wizard® SV lysis buffer.
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Total DNA
- Sample type:Rodent tailCultured cellsHuman/animal cells and tissues
- Target molecule:DNA
- Description:Wizard® SV genomic DNA purification system
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