DNA purification systems, Wizard® Plus SV minipreps
: Promega
The Wizard® Plus SV Minipreps DNA purification system is a silica membrane based system, provides a simple and reliable method for rapid isolation of plasmid DNA. Using the system, plasmid DNA can be purified from 1 to 10ml of overnight E.coli culture. The purified plasmid DNA can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation and also in vitro transcription reactions.
- Improved productivity – 20 minipreps processed in less than 45 minutes
- Consistent quality – alkaline protease step improves plasmid quality
- Performance – 1 to 20 μg of high quality plasmid DNA, enough for multiple applications
- No phenol extractions or precipitations required
- Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing
: The components in kit can be ordered separately.
: The complete kit includes Wizard® SV minicolumns, cell resuspension solution (CRA), cell lysis solution (CLA), neutralisation solution (NSB), alkaline protease solution, column wash solution (CWA), collection tubes and nuclease free water.
- Application:
- Method / Format:
- Refine target molecule:
- Sample type:
- Target molecule:
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