The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

• Maximises fluorescent signal by reducing cell wash artifacts
• Reduced well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput screens
• Mix-and-read protocol accelerates assay workflow and increases throughput
• Superior signal-to-noise ratio facilitates confirmation of endogenous or transiently transfected receptor activity during assay development
• Pre-optimised and validated protocols for the FLIPR® Tetra and FlexStation® Systems ensure navigation to both routine and unconventional cell lines and targets

FLIPR® Calcium 5 Assay Kit contains a novel fluorophore that amplifies the assay signal window while leveraging the superior masking technology used in the FLIPR® Calcium 4 Assay Kit to reduce background fluorescence. The kit is designed to work for the majority of GPCRs, including Chemokine and other difficult receptors, sticky compounds, allosteric modulators, as well as with calcium channels.